Home Shop RANKL (soluble) (human), (recombinant)

Available

RANKL (soluble) (human), (recombinant)

ALX-522-012

Datasheet
Manual
SDS
CofA

Already have an account?

RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG) is a potent inhibitor of RANKL.
Historical data has shown that the biological activity of RANKL supports the survival of dendritic cells and osteoclasts.

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

RANKL (soluble) (human), (recombinant) Schematic structure — **Figure 1:** *Schematic structure of human rhsRANKL (peptide, aa. 151-316).*

followed by a rabbit anti-mouse IgG-HRP at 1/1000 dilution for 30 minutes. OPD was used as a substrate for the peroxidase. Absorbance was measured at 490 nm in an ELISA reader.""""" — **Figure 2:** Binding of rhsRANKL to Osteoprotegerin. Recombinant, human sRANKL binds to Osteoprotegerin:Fc (see Prod. No. ALX-522-007).Method: Ligand binding assay: 96 well ELISA plates were coated O/N with 50 ng OPG-Fc (Prod. No. ALX-522-007) per well. After a blocking step

RANKL (soluble) (human), (recombinant) SDS-PAGE — SDS-PAGE Analysis of RANKL (soluble) (human), (recombinant). Lane 1: MW Marker, Lane 2: 1µg.

RANKL (soluble) (human), (recombinant) image — **Figure 3:** Mononuclear cells differentiate into Osteoclasts in the presence of M-CSF and RANKL, Soluble (human) (recombinant) (ALX-522-012). **Method:** Human CD14+ mononuclear cells isolated from adult peripheral blood were cultured in control medium (upper panel), in 25ng/ml M-CSF (middle panel), or in 25ng/ml M-CSF plus 50ng/ml RANKL (lower panel). Osteoclasts were identified as Tartrate-Resistant Acid Phosphatase (TRAP)-positive multinucleated cells. Osteoclasts were detected exclusively in presence of RANKL and in these culture conditions (M-CSF + RANKL), cells fused and generated multinucleated dark red TRAP-positive cells (arrows). Nuclei were stained with haematoxylin. Original magnification: 200x. *Picture courtesy of V.Kindler and D.Suvà, University of Geneva.*“””,RANKL (soluble) (human)

Product Details

Alternative Name	TRANCE, OPGL, ODF, TNFSF 11, CD254
Application Notes	ELISA: binds to RANK receptor.
Concentration	0.1mg/ml after reconstitution.
Endotoxin Content	<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Formulation	Lyophilized. Contains PBS.
MW	~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE).
Purity	≥90% (SDS-PAGE)
Reconstitution	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Solubility	Soluble in water.
Source	Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 152-317) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.
Specificity	Binds to human and mouse RANK.
Technical Info / Product Notes	FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	O14788

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

An in vitro model for discovery of osteoclast specific biomarkers towards identification of racehorses at risk for catastrophic fractures: G. Malek, et al.; Equine Vet. J. 1111, evj.13600 (2022), Abstract
Synthetic ligands of death receptor 5 display a cell-selective agonistic effect at different oligomerization levels: J. Beyrath, et al.; Oncotarget 7, 64942 (2016), Abstract — Full Text
Porous gelatin/tricalcium phosphate/genipin composites containing lumbrokinase for bone repair: Y.T. Fu, et al.; Bone 78, 15 (2015), Abstract
XPO1/CRM1-selective inhibitors of nuclear export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa): G.L. Gravina, et al.; J. Hematol. Oncol. 7, 46 (2014), Abstract — Full Text
Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation: Y.T. Fu, et al.; BMC Complement. Altern. Med. 14, 440 (2014), Abstract — Full Text
R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts: A. Miyamoto, et al.; Cytotechnology 64, 331 (2012), Abstract — Full Text
Crosstalk of osteoblast and osteoclast precursors on mineralized collagen–towards an in vitro model for bone remodeling: A. Bernhardt, et al.; J. Biomed. Mater. Res. A 95, 848 (2010), Abstract
Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture: K.M. Chen, et al.; Pharmazie 62, 388 (2007), Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004), Abstract
A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004), Abstract

Last modified: May 29, 2024

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

English (EU) SDS

If your language is not available please fill out the SDS request form

Your Cart

Your Cart

RANKL (soluble) (human), (recombinant)

ALX-522-012

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

Change Country

Tell us about You

Tell us about your paper

Tell us about You

Tell us about your project