MRP2 monoclonal antibody (M2III-5)

ALX-801-037

8 citations

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This antibody is covered by our Worry-Free Guarantee.

Product Details

Alternative Name	Multidrug resistance-associated protein 2, cMOAT, ABCC2
Application	ICC, IHC (FS), IHC (PS), WB
Application Notes	Immunocytochemistry: cytospin preparations Immunohistochemistry: Does not recognize mouse MRP2 in IHC.
Clone	M2III-5
Crossreactivity	Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5.
Formulation	Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
Host	Mouse
Immunogen	Recombinant C-terminal rat MRP2 (multidrug resistance-associated protein 2; cMOAT) (aa 1339-1541).
Isotype	IgG2b
Recommendation Dilutions/Conditions	Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.Immunohistochemistry (frozen sections, paraffin sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex or on paraffin sections after pretreatment with 0.01 M citric acid, pH 6.0 in distilled water at 100°C for 5 min 3 times. After washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase.Western Blot: Use 1:20-1:50 dilution and anti-mouse-HRP.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Species Reactivity	Human, Mouse, Rat
Specificity	Recognizes an internal epitope of MRP2.
UniProt ID	Q63120
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats.: Alaei Faradonbeh, F., Lastuvkova, H., et al.; Front. Physiol. 13, 859294 (2022), Reactant(s): Rat, Abstract
In Vitro Transport Activity and Trafficking of MRP2/ABCC2 Polymorphic Variants: Wen, X., Joy, M. S., et al.; Pharm. Res. 34, 1637 (2017), Abstract
Boldine enhances bile production in rats via osmotic and Farnesoid X receptor dependent mechanisms: J. Cermanova, et al.; Toxicol. Appl. Pharmacol. 285, 12 (2015), Application(s): Western Blotting, Immunohistochemistry, Abstract
Maternal bile acid transporter deficiency promotes neonatal demise: Y. Zhang, et al.; Nat. Commun. 6, 8186 (2015), Application(s): Immunoblot analysis of tissue homogenates, Abstract
Identification of the efflux transporter of the fluoroquinolone antibiotic ciprofloxacin in murine macrophages: studies with ciprofloxacin-resistant cells: Marquez, B., Caceres, N. E., et al.; Antimicrob. Agents Chemother. 53, 2410 (2009), Abstract
Analysis of the in vivo functions of Mrp3: M.G. Belinsky, et al.; Mol. Pharmacol. 68, 160 (2005), Abstract
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000), Abstract — Full Text
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science 271, 1126 (1996), Abstract