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COMP (rat):Angiopoietin-1 (human), (recombinant)

ALX-201-314

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ALX-201-314-C010 — 10 µg Available

$735.00

COMP (rat):Angiopoietin-1 (human), (recombinant) quantity
ALX-201-314-C050 — 50 µg Available

$1,840.00

COMP (rat):Angiopoietin-1 (human), (recombinant) quantity
ALX-201-314-C500 — 500 µg Inquire for Availability

$7,610.00

COMP (rat):Angiopoietin-1 (human), (recombinant) quantity

Soluble, stable and more potent Ang-1 variant.

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COMP (rat): Angiopoietin-1 (human), (recombinant) SDS-PAGE analysis — SDS-PAGE analysis of ALX-201-314: Lane 1: MW Marker, Lane 2: 1µg of COMP (rat):Angiopoietin-1 (human), (recombinant) protein.

COMP (rat):Angiopoietin-1 (human), (recombinant) Pentameric structure Transmission electron microscopy — **Figure 1:** COMP (rat):Angiopoietin-1 (human) (rec.) (Prod. No. ALX-201-314) forms mainly pentameric structures. **Method:** Glycerol spraying/low angle rotary metal-shadowed specimens were imaged by transmission electron microscopy.”””,COMP (rat):Angiopoietin-1 (human)

COMP (rat):Angiopoietin-1 (human), (recombinant) Structure Scheme — Structural scheme of ALX-201-314: Produced in CHO cells. The fibrinogen-like domain (aa 284-498) of human Ang-1 (angiopoietin-1) is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.

COMP (rat):Angiopoietin-1 (human), (recombinant) Immunoprecipitation — **Figure 2:** COMP (rat):Angiopoietin-1 (human) (rec.) (Prod. No. ALX-201-314) induces phosphorylation of Tie-2 in human umbilical vein endothelial cells.Method: Serum-starved human umbilical vein endothelial cells were treated with 200ng/ml of BSA (Co) or 200ng/ml of COMP:Angiopoietin-1 for 15 min. The phosporylation of Tie-2 (phospho-Tie-2) was measured after immunoprecipitation.”””,COMP (rat):Angiopoietin-1 (human)

Product Details

Alternative Name	COMP:Ang-1
Biological Activity	Induces phosphorylation of Tie-2 in primary cultured human and mouse vascular endothelial cells. More potent than native Ang-1.
Concentration	~0.2-0.4 mg/mL(Please refer to Certificate of Analysis for lot-specific concentration.)
Endotoxin Content	<0.1EU/µg protein (LAL test; BioWhittaker)
Formulation	Liquid. 0.2µm-filtered solution in PBS containing 0.01% CHAPS.
Purity	≥90% (SDS-PAGE)
Source	Produced in CHO cells. The fibrinogen-like domain (aa 284-498) of human Ang-1 (angiopoietin-1) is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.
Specificity	Binds to human Tie-2.
Technical Info / Product Notes	FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	Q15389 (human Angiopoietin-1), P35444 (rat COMP)

Handling & Storage

Use/Stability	Working aliquots are stable for up to 3 months when stored at -80°C.
Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization: J.M. Ha, et al.; Exp. Mol. Med. 54, 1133 (2022), Abstract
Novel TIE-2 inhibitor BAY-826 displays in vivo efficacy in experimental syngeneic murine glioma models: H. Schneider, et al.; J. Neurochem. 140, 170 (2017), Abstract
Angiopoietin-1 deficiency increases tumor metastasis in mice: I.P. Michael, et al.; BMC Cancer 17, 539 (2017), Abstract — Full Text
COMP-Ang1 prevents periodontitic damages and enhances mandible bone growth in an experimental animal model: G. Bhattarai, et al.; Bone 92, 168 (2016), Abstract
COMP-Ang1 enhances DNA synthesis and cell cycle progression in human periodontal ligament cells via Tie2-mediated phosphorylation of PI3K/Akt and MAPKs: S.S. Lim, et al.; Mol. Cell. Biochem. 416, 157 (2016), Abstract
Cartilage oligomeric matrix protein enhances the vascularization of acellular nerves: W.L. Cui, et al.; Neural Regen. Res. 11, 512 (2016), Abstract — Full Text
Cartilage Oligomeric Matrix Protein Angiopoeitin-1 Provides Benefits During Nerve Regeneration In Vivo and In Vitro: L. Qiu, et al.; Ann. Biomed. Eng. 43, 2924 (2015), Abstract — Full Text
Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats: K. Kawamura, et al.; Plos One 9, e98639 (2014), Abstract — Full Text
Angiopoietin-1 promotes LYVE-1-positive lymphatic vessel formation: T. Morisada, et al.; Blood 105, 4649 (2005), Abstract
Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2: P. Saharinen, et al.; J. Cell Biol. 169, 239 (2005), Abstract

Last modified: May 29, 2024

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COMP (rat):Angiopoietin-1 (human), (recombinant)

ALX-201-314

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