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Protein Analysis
What exactly does the kit ADI-EKS-895 detect?
The kit ADI-EKS-895 is specific for human Hsp90a and will not react with human Hsp90ß. Human Hsp90a and Hsp90ß are…
Tue, Jul 18, 2023
How were the <i>E.coli</i> host cell protein standards generated?
Most of the E.coli strains used in the industry and in the production of biologics are derived from K12. The…
Tue, Jul 18, 2023
Do you sell a positive control for ADI-900-175, Serotonin ELISA kit, or could you advice on a suitable one?
We do not provide positive control samples with the kit. If you need to ensure accurate recovery of Serotonin from…
Tue, Jul 18, 2023
Which type of plasma sample do you recommend for using with ADI-900-175?
The kit was validated with citrate plasma samples. EDTA plasma will likely work fine as long as the final EDTA…
Tue, Jul 18, 2023
I have different cell lysates which I would like to run with ADI-EKS-895, HSP90a (human) ELISA kit, but I am not sure if the formulation of my lysis buffer is identical to the extraction buffer present in the kit. Will I be able to analyze my samples
The exact formulation of our extraction buffer is proprietary and cannot be disclosed. The buffer is optimized to achieve complete…
Tue, Jul 18, 2023
Are the polyclonal antibodies against E.coli, CHO, and HEK293T host cell proteins purified and by what method?
These antibodies were indeed purified using MabSelect™ (Protein A affinity column) from GE Healthcare.
Tue, Jul 18, 2023
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
I plan to use your ADI-900-020A kit to measure Endothelin-1 (ET-1) concentration in cell culture supernatant. Samples contain 10% fetal bovine serum (FBS). Will I need to extract them?
The ET-1 ELISA kit is suitable for use with tissue culture supernatants containing up to 10% FBS. In our spike…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023
Can the Serotonin ELISA kit, ADI-900-175, be used with samples from any species?
Serotonin is conserved across all species and the antibody provided in the kit will bind to Serotonin independently from the…
Tue, Jul 18, 2023