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Protein Analysis
Liver lysate samples when probed with HO-1 polyclonal antibody, ADI-SPA-896, shows two bands on western blot. What is the possible explanation as to why two bands detected?
Western blot image displayed on product page with ADI-SPA-896 shows two bands being detected. One of them is full length…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023
What is the difference between Z-VAD(OMe)-FMK (BML-P416) and Z-VAD-FMK (ALX-260-020)? Which Caspase inhibitor is best for cell based applications? Which inhibitor works in cell free assays?
Z-VAD(OMe)-FMK (BML-P416) has methyl ester group making this irreversible caspase inhibitor cell permeable and therefore is used in cell based…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023
Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023
Why is a big change in OD not noticed in an inhibitor screening assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535?
Tyrosinase reaction rates reaches maximal velocity in less than two minutes where an initial burst phase is followed by steady-state…
Tue, Jul 18, 2023
What specific tips are useful for substrate preparation with Tyrosinase (human), (recombinant) (His-tag), BML-SE535 assay?
When preparaing an assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535, please consider that Substrate-1, MBTH (3-methyl-2-benzothiazolinone hydrazone) is unstable. Make…
Tue, Jul 18, 2023
Can results from SARS-CoV-2 IgG ELISA Kit ENZ-KIT170 be correlated to plaque-forming units (pfu/ml) of SARS-CoV-2?
SARS-CoV-2 IgG ELISA Kit is designed for the qualitative measurement of the human SARS-CoV-2 IgG. ELISA detects human antibodies to COVID. …
Tue, Jul 18, 2023
Is charcoal-stripped FBS and serum free DMEM required for spike and recovery experiment with cell culture sample with Progesterone ELISA kit, ADI-900-011?
ANS: We suggest to prepare standard curve in the same unconditioned media used to culture samples. General recommendation for diluting…
Tue, Jul 18, 2023