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Protein Analysis
What are important critical factors for success while working with Protein A ELISA kit, ADI-900-057
Following are two critical factors to be considered: 1. If samples contain IgG, standards must be boiled. 2. Diluted standards…
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023
Please provide the amino acid sequence of the FLAG tag for Irisin (human),(recombinant)(active), ADI-908-307.
FLAG® is a 8 amino acid sequence DYKDDDDK. In our Irisin (human), (recombinant) (active) (ADI-908-307) amino acids sequence 32-143 of…
Tue, Jul 18, 2023
How is Melanoma marker (human) monoclonal antibody (HMB45), ENZ-C34930 purified from ascites?
Melanoma marker (human) monoclonal antibody (HMB45) (ENZ-C34930) is purified following ammonium sulphate precipitation of ascites. Supernatant is further purified on…
Tue, Jul 18, 2023
Can a rough estimate antibody concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1 be stated?
The supernatant of ammonium sulfate precipitate hybridoma tissue culture supernatant for The a-Gal epitope monoclonal antibody (ALX-801-090-1) is concentrated and…
Tue, Jul 18, 2023
What is the concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1?
The a-Gal epitope monoclonal antibody (ALX-801-090-1) is offered as partially purified ammonium sulfate precipitate tissue culture supernatant. The volume provided…
Tue, Jul 18, 2023
What does Standard Diluent refer to in several ELISA user manual when cell culture supernatant is used?
Standard diluent refers to either the tissue culture media (a.k.a. non-conditioned media) or the assay buffer provided. So, the supernatant…
Tue, Jul 18, 2023
Can Sample diluent (80-1504) be used to dilute antibody if one runs out of antibody diluent (80-1759) with HO-1 (human), ELISA kit, ADI-EKS-800?
We do not recommend using Sample diluent (80-1504) to dilute antibody instead of antibody diluent (80-1759) with HO-1 (human), ELISA…
Tue, Jul 18, 2023
What information does Lot specific Certificate of Analysis for Protein A ELIA kit, ADI-900-057 have?
Lot specific Certificate of Analysis for Protein A ELISA kit (ADI-900-057) lists: assay range, OD values for High, Medium, Low,…
Tue, Jul 18, 2023
Where do I find validation data for Protein A ELISA kit, ADI-900-057 since this is not listed on datasheet?
Description of Validation experiments and the data for Protein A ELISA kit (ADI-900-057) are listed in pages from 9-11 of…
Tue, Jul 18, 2023