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What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
What are the differences between TRAIL, KillerTRAIL™ and SuperKillerTRAIL™?
To induce apoptosis, TRAIL needs to form multimers, showing increasing activity with increasing multimerization levels. Multimerization of TRAIL can be…
Tue, Jul 18, 2023
I am working with rabbit sera. Can I use your Oxytocin ELISA kit for detecting oxytocin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023
What protocol can be used for drying samples for analysis with the Oxytocin and Arg8-Vasopressin ELISA kits?
Samples can be dried using either vacuum centrifugation (e.g., SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
Is this Vasopressin ELISA kit species-independent?
The vasopressin molecule sequence does not vary between mammalian species making this kit species-independent. Cited sample origins include dog, human,…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
What is the amount or concentration of SIRT1 provided in BML-AK555?
The volume or concentration of SIRT1 is lot-dependent. Each kit contains 100 units of the enzyme. Concentration on specific lot of SIRT1 is available…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
Does the standard included in BML-AK555 need to be incubated with recombinant SIRT1?
The deacetylated standard included in the kit can be used to create a standard curve giving the relationship between deacetylated…
Tue, Jul 18, 2023