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Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small…
Tue, Jul 18, 2023
What is actually being stained in the nucleolus by the Nucleolar-ID™ Green Detection Reagent?
We use an RNA binding dye and this has been validated. However, in the context of live cells we cannot…
Tue, Jul 18, 2023
Detection Methods for Immunoassays
The immunological part of an immunoassay ends typically with either a wash or separation step. In these steps, any unbound…
Tue, Jul 18, 2023
For the ROS/RNS detection kit, ENZ-51001, can samples be stored after the final wash buffer step in the protocol, and do the microscopy at a later date?
No, this is a vital stain. Live cells must be read immediately per the manual.
Tue, Jul 18, 2023
Poor Standard Curve
What was used as the standard diluent? Diluents other than the supplied assay buffer may contain interfering substances that can…
Tue, Jul 18, 2023
Poor Precision
Were the wells washed properly? All wells receive the same treatment during the wash step. If some are washed less…
Tue, Jul 18, 2023
Drift
Were reagents brought to room temperature prior to use? If the reagents are not at a constant temperature prior to…
Tue, Jul 18, 2023
Weak Color Development
Was substrate added at the correct point in the assay? See the assay procedure provided in the instruction manual. Were…
Tue, Jul 18, 2023
What is the Kd and quantum yield of FluoForte® Calcium Assay kit?
Kd is estimated to be 389 nM (22°C, pH 7.0 –7.5). Maximum quantum yield is 0.18 at pH 7.2.
Tue, Jul 18, 2023
How do the ROS/RNS dyes work?
Nitric Oxide (Red dye): The strategy for fluorescence-based detection of NO employs an o-phenylenediamine scaffold, which in the presence of…
Tue, Jul 18, 2023