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Non-Antibody Based Assays
Activity Assays Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring…
Tue, Jul 18, 2023
Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023
Indirect Immunoassays
< The enzyme-linked immunosorbent assay (ELISA), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023
Antibody Conjugation
Abbreviation Full Name PE R-Phycoerythrin DY DYTM is a trademark of the Dyomics , Inc. DyLightTM 488 …
Tue, Jul 18, 2023
General Abbreviations
Abbreviation Full Name mAb Monoclonal Antibody pAb Polyclonal Antibody cAb Clonal Antibody CT C-terminus NT …
Tue, Jul 18, 2023
What storage conditions are needed for an antibody?
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage recommendations are listed on the…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023
What kind of instrumentation is required to read a CLIA assay?
A plate luminometer is necessary to read the RLU (relative light units) generated by these assays. It is not necessary…
Tue, Jul 18, 2023
When is it appropriate to use a CLIA instead of an EIA?
A CLIA, or chemiluminescent immunoassay, is more sensitive than an EIA (enzyme immunoassay) and is used when samples contain very…
Tue, Jul 18, 2023