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Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
EGS (ethyleneglycol-bis-succinimidyl-succinate) Fixation
EGS (ethyleneglycol-bis-succinimidyl-succinate) fixation (method to preserve microtubules and membrane bound antigens) Rinse cells with pre-warmed (37°C) PBS. Prepare EGS stock…
Tue, Jul 18, 2023
Formaldehyde Fixation
Formaldehyde fixation (for membrane associated components) Fixative Preparation Dissolve 16 g paraformaldehyde in 80 ml dH2O by stirring at 70°C…
Tue, Jul 18, 2023
Methanol Fixation
Methanol fixation (for cytoskeletal components) The methanol fixation is an easy method. However, it frequently solubilizes and removes membrane bound…
Tue, Jul 18, 2023
Checking for Interference or Linearity
Interference is when some component of a sample prevents the appropriate binding events to take place. Percent recovery is the…
Tue, Jul 18, 2023
Immunohistochemistry Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membrane Preparation Protocol
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membranes ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Cell ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note:The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not been…
Tue, Jul 18, 2023