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Can samples diluted with the Assay Buffer from the cAMP Direct ELISA kit be used with the cGMP Direct ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the end…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP complete ELISA kit be used with the cGMP complete ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these ELISA kits?
No, these are colorimetric ELISA kits for the quantification of extra-cellular cAMP/cGMP in culture supernatants, serum, and saliva from any…
Tue, Jul 18, 2023
Intracellular Staining Protocol: Two Step Fix/Perm
Two Step Fixation and Permeabilization Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation…
Tue, Jul 18, 2023
Intracellular Staining Protocol: One Step Fix/Perm
One Step Fixation and Permeabilization Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation/Permeabilization…
Tue, Jul 18, 2023
Extracellular Staining Protocol
Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation Buffer, ADI-950-011 PBS (Phosphate Buffered…
Tue, Jul 18, 2023
Fixation of the Cytoskeleton
Fixation of the cytoskeleton If components of the cytoskeleton are of interest, cells can be extracted prior to fixation. Rinse…
Tue, Jul 18, 2023
Blue nuclear counterstain signal is dramatically reduced in treated cells.
If the blue nuclear signal decreases by more than 30%, the testing compound is generally cytotoxic. Optimize the concentration of…
Tue, Jul 18, 2023
After incubation with the dual detection reagent at 37° C for 30 min, the cells were not healthy and rounding up. Some cells were also detached from the plate. No specific staining on positive control was observed vs. DMSO controls.
For fragile cells, the following modification to the standard protocol are suggested: Use PBS/5% FBS as an assay buffer or…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these Complete ELISA kits?
These are colorimetric ELISA kits for the quantification of both intra- and extra-cellular cAMP/cGMP in cell lysates, culture supernatants, serum,…
Tue, Jul 18, 2023