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Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023
My cell culture samples are in DMEM. Will this interfere with the Nitric Oxide assay?
Yes. The nitrate salt used in this particular media will cause interference. It is important to check the published media…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023
I am using the Direct 8-iso PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
At this point, you have added HCl to NaOH which will yield NaCl + H2O. There is little buffering capacity…
Tue, Jul 18, 2023
Do eicosanoid assays require an extraction?
The majority of samples do not require an extraction. However, in some situations, an extraction will be required when the…
Tue, Jul 18, 2023
What antibody pairs are used in the kit?
This varies from kit to kit. Refer to the product specific instruction manual for more information.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I prepare a different standard range than what is recommended in the protocol?
You may prepare a standard range to suit your specific needs. However, the dynamic range has already been optimized, and…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, should I store the coated plates wet or dry?
You may store the coated plates for short periods of time (2-3 days) in blocking buffer, being careful to seal…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will my plate coating technique affect the precision of my plates and the overall assay?
It is possible for plate coating technique to lead to poor precision. Please refer to the insert for specific recommendations.
Tue, Jul 18, 2023