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Inconsistent results between PDI assay experiments.
This is caused by inappropriate Stop Reagent addition. Be sure to pre-incubate with Stop Reagent to terminate both the enzyme…
Tue, Jul 18, 2023
Glossary
Antibody: An immunoglobulin that recognizes some portion of an antigen molecule. The antibody contains a species-specific Fc region and antigen-specific…
Tue, Jul 18, 2023
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
What is the purpose of treating samples with 5% metaphosphoric acid (MPA)?
MPA should be added to fresh samples prior to sample handling or sample storage. That is required to deproteinize samples…
Tue, Jul 18, 2023
What is the principle of the Glutathione (GSSG/GSH) assay?
The Glutathione (total), detection kit is a complete kit that allows the determination of total, reduced, and oxidized glutathione concentrations…
Tue, Jul 18, 2023
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
What can interfere with the assay?
Reducing agents (such as ascorbic acid, beta-mercaptoethanol, dithiothreitol, and cysteine) and thiol reactive compounds (such as maleimides) are known to…
Tue, Jul 18, 2023
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
The following kits are only suitable for live cell staining only. Use them only for live cell analysis. Cyto-ID® Autophagy…
Tue, Jul 18, 2023
Poor staining is observed in the positive control.
Stained reagents have been exposed to strong light. Protect samples from exposure to strong light and analyze them immediately after…
Tue, Jul 18, 2023