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How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
Are the immunogens used to produce anti-HCPs antibodiesin the CHO host cell protein ELISA kit (ENZ-KIT128) derived from the cell culture supernatant or extracted proteins from CHO cells?
The antigens used to produce anti-HCPs antibodies for CHO host cell protein ELISA kit, ENZ-KIT128 are purified from the conditioned…
Tue, Jul 18, 2023
What could be the reasons for lower spike and recovery results with Protein A ELISA kit, ADI-900-057?
There are several reasons for lower spike and recovery return values with Protein A ELISA kit (ADI-900-057). One possible reason…
Tue, Jul 18, 2023
Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023
Is Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 compatible with anti-fluorescence quenching agent and fluoromount-G?
The CYTO-ID® Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 kits is compatible with anti-fluorescence quenching agent and Fluoromount-G.
Tue, Jul 18, 2023
Can the microplate be read following addition of ROS/Superoxide Detection Solution with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010?
Yes, plates can be read using bottom reading option without removing the ROS/Superoxide Detection Solution and without washing.
Tue, Jul 18, 2023
How do we perform QC for E. coli host cell protein ELISA kit, ENZ-KIT127?
Quality control for E. coli host cell protein ELISA kit (ENZ-KIT127) is performed by randomly selecting multiple plates from each…
Tue, Jul 18, 2023
Buffer salts in 10x PBS-T wash buffer in E. coli host cell protein ELISA kit, ENZ-KIT127 has precipitated upon storage at 4°C. Is this usual and how to dissolve the salts?
On occasions, buffer salts precipitate out of solution at 4°C. Warming the bottle containing buffer at 37°C and frequent vortexing would…
Tue, Jul 18, 2023
What is the difference is between Microycystin-LR (Analytical Standard), ALX-350-431 and Microcystin-LR, ALX-350-012?
Microycystin-LR (Analytical Standard), ALX-350-431-C010 and Microcystin-LR, ALX-350-012 differ in percentage purity. Microcystin-LR (Analytical Standard), ALX-350-431-C010 is =99% (HPLC). Microcystin-LR, ALX-350-012…
Tue, Jul 18, 2023