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How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
I have always seen the TMB Substrate Solution as a clear solution, but today the solution had significant green-blue tinge. What might have caused this color change?
The color tinge in the TMB substrate is due to contamination by the conjugate. To avoid contamination it is important…
Tue, Jul 18, 2023
Does the kit work for species other than human?
The ADI-EKS-390A kit is actually species independent; therefore, as long as there is sufficient PKA activity in your samples and…
Tue, Jul 18, 2023
Can I use this kit to measure the concentration of PKA in my samples?
The kit does not measure the concentration of PKA in samples. It is intended for looking at relative PKA kinase activity.
Tue, Jul 18, 2023
It appears that the positive control is not working properly. What could be the reason?
The nature of that protein is that it can quickly degrade unless it is handled very carefully. It must be…
Tue, Jul 18, 2023
What is the positive control provided in the kit?
The positive control provided in the kit ADI-EKS-390A is recombinant human PKAc beta (or catalytic subunit C-beta of PKA) expressed…
Tue, Jul 18, 2023
What is the principle of the PKA kinase activity assay?
The PKA kinase activity kit allows relative quantification of PKA kinase activity in different sample types (purified, partially purified and…
Tue, Jul 18, 2023