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What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Can the cells be trypsinized to determine protein aggregation in cell based assay for testing drug responsiveness?
Trypsin can be used with ProteoStat® Aggresome detection kit. Following lysis, detergent can be added to permeabilize the cells. The…
Tue, Jul 18, 2023
Can cell lysates be used to determine protein aggregation with ProteoStat® Aggresome detection reagent?
Working with cell lysates would be challenging, because detergents can cause high background. It is possible to titrate the amount…
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023
Can ADI-EKS-750 detect human antibodies against DnaK, an E.coli analog of HSP70?
A similar assay has not been performed and therefore, a positive outcome of such experiment cannot be guaranteed. We would suggest using…
Tue, Jul 18, 2023
Is ADI-EKS-725A specific for HSP70B’ or does it also detect HSP70?
This kit was developed with the sole purpose to detect and quantify HSP70B’ in cell lysates, serum and tissue of…
Tue, Jul 18, 2023
Is HSP70B’; simply a variant of HSP70?
HSP70B’ is a product of a separate gene, which is devoid of introns similarly to HSP70. The HSP70B’ has 77%…
Tue, Jul 18, 2023
Can I detect HSP70 in urine with ADI-EKS-700B, ADI-EKS-715, or ENZ-KIT-101?
Urine is not a sample type which has been validated for these kits, nor are there any publications found citing usage…
Tue, Jul 18, 2023
How stable are my samples prior to HSP70 quantification?
Different storage conditions for samples have not been analyzed or compared. If samples have been stored at -20°C or below…
Tue, Jul 18, 2023