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What is a good positive control to study protein aggregation in cells?
MG132 treated cells would serve as positive control to determine background noise compared to signal along with untreated negative control.
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023
What is the suggested remedy to minimize signal in the FITC emission filter while co-staining other proteins?
The excitation and emission spectra of the dye is provided on page 12 of the kit manual. The spectrum indicates…
Tue, Jul 18, 2023
Can the FluoForte® Calcium assay kit be used to monitor Ca2+ flux in nicotinic acetylcholine receptors on Flexstation. Can the FluoForte dye monitor Ca2+ in our model system given that FluoForte dye has been validated only in GPCR signaling path
While no specific references are available for application of FluoForte to assay nicotinic receptor, there are numerous publications regarding the…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023
Can ADI-EKS-750 detect human antibodies against DnaK, an E.coli analog of HSP70?
A similar assay has not been performed and therefore, a positive outcome of such experiment cannot be guaranteed. We would suggest using…
Tue, Jul 18, 2023
Is ADI-EKS-725A specific for HSP70B’ or does it also detect HSP70?
This kit was developed with the sole purpose to detect and quantify HSP70B’ in cell lysates, serum and tissue of…
Tue, Jul 18, 2023
Is HSP70B’; simply a variant of HSP70?
HSP70B’ is a product of a separate gene, which is devoid of introns similarly to HSP70. The HSP70B’ has 77%…
Tue, Jul 18, 2023
Can I detect HSP70 in urine with ADI-EKS-700B, ADI-EKS-715, or ENZ-KIT-101?
Urine is not a sample type which has been validated for these kits, nor are there any publications found citing usage…
Tue, Jul 18, 2023
How stable are my samples prior to HSP70 quantification?
Different storage conditions for samples have not been analyzed or compared. If samples have been stored at -20°C or below…
Tue, Jul 18, 2023