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27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023
Why is a big change in OD not noticed in an inhibitor screening assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535?
Tyrosinase reaction rates reaches maximal velocity in less than two minutes where an initial burst phase is followed by steady-state…
Tue, Jul 18, 2023
What is the difference between Z-VAD(OMe)-FMK (BML-P416) and Z-VAD-FMK (ALX-260-020)? Which Caspase inhibitor is best for cell based applications? Which inhibitor works in cell free assays?
Z-VAD(OMe)-FMK (BML-P416) has methyl ester group making this irreversible caspase inhibitor cell permeable and therefore is used in cell based…
Tue, Jul 18, 2023
Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023
Can PAP-AMC, Tyrosinase substrate, BML-EI405 function in live cell assay?
PAP-AMC, Tyrosinase substrate, BML-EI405 has been used in vitro assays. We do not have information if this substrate works efficiently…
Tue, Jul 18, 2023
What specific tips are useful for substrate preparation with Tyrosinase (human), (recombinant) (His-tag), BML-SE535 assay?
When preparaing an assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535, please consider that Substrate-1, MBTH (3-methyl-2-benzothiazolinone hydrazone) is unstable. Make…
Tue, Jul 18, 2023
What is the formulation of BIOMOL® Green (BML-AK111)?
The formulation of BIOMOL® (BML-AK111) Green is proprietary.
Tue, Jul 18, 2023
Does ORGANELLE-ID-RGB® III assay kit, ENZ-51032, bind to a target protein in ER and Golgi compartment?
The Golgi-green dye in the ORGANELLE-ID-RGB® III assay kit (ENZ-51032) is a fluorescent lipid analog which is transported directly into…
Tue, Jul 18, 2023