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Life Sciences
Immunohistochemistry Protocol
Protocols for immunohistochemistry vary widely due to differences in antigens and their recognition by antibodies. Various fixation methods can uncover…
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
What storage conditions are needed for an antibody?
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage recommendations are listed on the…
Tue, Jul 18, 2023
General Abbreviations
Abbreviation Full Name mAb Monoclonal Antibody pAb Polyclonal Antibody cAb Clonal Antibody CT C-terminus NT …
Tue, Jul 18, 2023
Non-Antibody Based Assays
Activity Assays Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring…
Tue, Jul 18, 2023
Indirect Immunoassays
The enzyme-linked immunosorbent assay (ELISA Kits), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023
What is the best way to analyze my data? What is 4-PL curve-fitting?
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of…
Tue, Jul 18, 2023
What kind of instrumentation is required to read a CLIA assay?
A plate luminometer is necessary to read the RLU (relative light units) generated by these assays. It is not necessary…
Tue, Jul 18, 2023