Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Life Sciences
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
What can interfere with the assay?
Reducing agents (such as ascorbic acid, beta-mercaptoethanol, dithiothreitol, and cysteine) and thiol reactive compounds (such as maleimides) are known to…
Tue, Jul 18, 2023
Can whole blood samples be used?
High background may result from the presence of hemoglobin in your sample. Hemoglobin (Hb) has a significant absorption at 340…
Tue, Jul 18, 2023
Glossary
Antibody: An immunoglobulin that recognizes some portion of an antigen molecule. The antibody contains a species-specific Fc region and antigen-specific…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023
Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023