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Life Sciences
Are the immunogens used to produce anti-HCPs antibodiesin the CHO host cell protein ELISA kit (ENZ-KIT128) derived from the cell culture supernatant or extracted proteins from CHO cells?
The antigens used to produce anti-HCPs antibodies for CHO host cell protein ELISA kit, ENZ-KIT128 are purified from the conditioned…
Tue, Jul 18, 2023
Can test compound dissolved in DMSO be used in cell based assay to measure Reactive Oxygen Species with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010
We cannot confirm if dissolving the compound in DMSO could affect the assay, since DMSO could inhibit hydroxyl radical generation…
Tue, Jul 18, 2023
What is the lot-to-lot variation for CHO host cell protein ELISA kit, ENZ-KIT128?
In general, the antibodies provided with CHO host cell protein ELISA kit (ENZ-KIT12)8 are stable and we do not change…
Tue, Jul 18, 2023
Does PACOCF3, BML-ST336 inhibit all isoforms of phospholipase A2 (PLA2)?
PACOCF3 (BML-ST336) inhibits of both Ca2+-dependent cytosolic cPLA2 (IC50=45 µM) and Ca2+-independent phospholipases A2 iPLA2 (IC50=3.8 µM). Unfortunately, we have…
Tue, Jul 18, 2023
Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023
What are the Intra- assay and Inter-assay precision percent coefficient of variation for the concentrations between lots for E. coli host cell protein ELISA kit, ENZ-KIT127 ?
Concerning the E. coli host cell protein ELISA kit (ENZ-KIT127) our intra-assay %CV is
Tue, Jul 18, 2023
Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
What could be the reason for low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001?
If observing a low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay (ENZ-61001), please consider the following. The…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023
What is the concentration of the PGE2-Alkaline phosphatase conjugate in the PGE2 ELISA kit, ADI-900-001?
Concentration of the PGE2-AP conjugate is proprietary and can't be revealed.
Tue, Jul 18, 2023