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Life Sciences
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023
How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
When doing a four-color IHC staining, is there a risk that the colors will fade during the process?
It is recommended to optimize with each step in order to avoid fading of each color due to time-lag between…
Tue, Jul 18, 2023
I have always seen the TMB Substrate Solution as a clear solution, but today the solution had significant green-blue tinge. What might have caused this color change?
The color tinge in the TMB substrate is due to contamination by the conjugate. To avoid contamination it is important…
Tue, Jul 18, 2023
Does the product ADI-EKS-800, HO-1 (human), ELISA kit also work for human plasma and serum?
The kit has been validated for the measurement of intracellular HO-1 in cell lysates and tissue extracts and the protocol refers…
Tue, Jul 18, 2023
Will the NGAL (human) ELISA kit also work with species other than human?
This kit has been solely developed and validated for human samples. Please note however that Enzo has several other NGAL ELISA kits…
Tue, Jul 18, 2023
How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023
How should urine samples be prepared for use with the NGAL (human) ELISA kit?
Urine preparation usually only requires centrifugation if there is inflammation or infection of the urinary tract (to remove polymorphonuclear neutrophils…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023