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Life Sciences
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
Can ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, be used for microplate assay format?
ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, has not been validated in microplate format. The kit is validated for fluorescence microscopy and…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
What is the detection mechanism used in your cellular senescence activity assay (ENZ-KIT129)?
Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can…
Tue, Jul 18, 2023
Are ENZ-KIT129 and ENZ-KIT130 compatible with any cell line?
The SA-b-galactosidase is a universal marker for any senescent cell and this assay is not cell type-specific.
Tue, Jul 18, 2023
What is the purpose of the cell pre-treatment solution in ENZ-KIT130?
There are two main types of b-galactosidases in senescent cells: SA-b-galactosidase and lysosomal b-galactosidase. The latter can only be detected…
Tue, Jul 18, 2023
How long can the cells be saved until analysis by flow cytometry when using the Cellular senescence live cell analysis assay (ENZ-KIT130)?
Analyzing the results within a few hours is recommended because this assay uses live cells. If the cells lyse, the dye…
Tue, Jul 18, 2023