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Life Sciences
How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023
How are Quality control tests for each lot of E.coli host cell protein ELISA kit, ENZ-KIT127 performed?
Quality control testing is performed by randomly selecting multiple plates from each lot. All lot-specific components are tested together using…
Tue, Jul 18, 2023
How can staining be improved with the PROTEOSTAT® Aggresome detection kit, ENZ-51035?
Optimize the dilution of the dye provided with PROTEOSTAT® Aggresome detection kit (ENZ-51035) to improve staining. Also incubationb time can…
Tue, Jul 18, 2023
How is the cross reactivity for 17ß-Estradiol related steroid compounds determined with 17ß-Estradiol ELISA kit, ADI-900-008?
During the develomment of 17ß-Estradiol ELISA kit (ADI-900-008), the cross reactivities for a number of related steroid compounds was determined…
Tue, Jul 18, 2023
What starting volume is required for COX activity assay kit, ADI-907-003?
For COX activity assay kit, ADI-907-003 we recommend starting volumes of 50 µL COX-I or COX-II preparations. Actual sample volumes required…
Tue, Jul 18, 2023
Can some guidance be provided to detect the activity of COX1 and COX2 from human plasma, and advise assay design with the Ibuprofen, meloxicam, known COX-2 inhibitors for COX activity assay kit, ADI-907-003?
We have not specifically validated this kit with human plasma samples. Page 5 of user manual provides the work flow. This provides…
Tue, Jul 18, 2023
How does EFLUXX-ID® Green multidrug resistance assay kit, ENZ-51029 and EFLUXX-ID® Gold multidrug resistance assay kit, ENZ-51030 detect activity of transporter proteins?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound…
Tue, Jul 18, 2023
How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023