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ELISA Immunoassays
How should I store reconstituted standard, antibody and conjugatefor ADI-900-175?
The standard, antibody and conjugate are single use items and should to be stored at -20°C. Any leftover of reconstituted…
Tue, Jul 18, 2023
How were the CHO host cell protein standards generated?
The CHO host cell protein standards were prepared from multiple CHO K1 cell lines growing in suspension and in chemically…
Tue, Jul 18, 2023
Are the polyclonal antibodies against <i>E.coli</i>, CHO, and HEK293T host cell proteins purified and by what method?
These antibodies were indeed purified using MabSelect™ (Protein A affinity column) from GE Healthcare.
Tue, Jul 18, 2023
What exactly does the kit ADI-EKS-895 detect?
The kit ADI-EKS-895 is specific for human Hsp90a and will not react with human Hsp90ß. Human Hsp90a and Hsp90ß are…
Tue, Jul 18, 2023
Do you sell a positive control for ADI-900-175, Serotonin ELISA kit, or could you advice on a suitable one?
We do not provide positive control samples with the kit. If you need to ensure accurate recovery of Serotonin from…
Tue, Jul 18, 2023
Can I use the kit ADI-EKS-895 to measure HSP90a activity?
ADI-EKS-895 is used to measure the total amount of human HSP90a in a given sample. It cannot be used for…
Tue, Jul 18, 2023
How were the <i>E.coli</i> host cell protein standards generated?
Most of the E.coli strains used in the industry and in the production of biologics are derived from K12. The…
Tue, Jul 18, 2023
The sensitivity of ADI-900-020A is listed as 0.41 pg/mL, whereas the standard range only goes as low as 0.78 pg/mL. What is the limit of detection of the assay?
Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero…
Tue, Jul 18, 2023
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023