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ELISA Immunoassays
Which type of plasma sample do you recommend for using with ADI-900-175?
The kit was validated with citrate plasma samples. EDTA plasma will likely work fine as long as the final EDTA…
Tue, Jul 18, 2023
Do you sell a positive control for ADI-900-175, Serotonin ELISA kit, or could you advice on a suitable one?
We do not provide positive control samples with the kit. If you need to ensure accurate recovery of Serotonin from…
Tue, Jul 18, 2023
Can the ADI-EKS-895 be used to measure mouse HSP90a?
No, the capture antibody does not react with mouse Hsp90a, which prevents the assay from being able to recognize those…
Tue, Jul 18, 2023
Are the polyclonal antibodies against <i>E.coli</i>, CHO, and HEK293T host cell proteins purified and by what method?
These antibodies were indeed purified using MabSelect™ (Protein A affinity column) from GE Healthcare.
Tue, Jul 18, 2023
What exactly does the kit ADI-EKS-895 detect?
The kit ADI-EKS-895 is specific for human Hsp90a and will not react with human Hsp90ß. Human Hsp90a and Hsp90ß are…
Tue, Jul 18, 2023
I have different cell lysates which I would like to run with ADI-EKS-895, HSP90a (human) ELISA kit, but I am not sure if the formulation of my lysis buffer is identical to the extraction buffer present in the kit. Will I be able to analyze my samples
The exact formulation of our extraction buffer is proprietary and cannot be disclosed. The buffer is optimized to achieve complete…
Tue, Jul 18, 2023
How were the CHO host cell protein standards generated?
The CHO host cell protein standards were prepared from multiple CHO K1 cell lines growing in suspension and in chemically…
Tue, Jul 18, 2023
The sensitivity of ADI-900-020A is listed as 0.41 pg/mL, whereas the standard range only goes as low as 0.78 pg/mL. What is the limit of detection of the assay?
Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero…
Tue, Jul 18, 2023
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023