ELISA Immunoassays

Is there a requirement for bridging when changing lots of E. coli host cell protein ELISA kit, ENZ-KIT127?
A bridging study is required if this is a part of defined or specified SOP for a facility or a…
Tue, Jul 18, 2023

How are Quality control tests for each lot of E.coli host cell protein ELISA kit, ENZ-KIT127 performed?
Quality control testing is performed by randomly selecting multiple plates from each lot. All lot-specific components are tested together using…
Tue, Jul 18, 2023

How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023

How is the cross reactivity for 17ß-Estradiol related steroid compounds determined with 17ß-Estradiol ELISA kit, ADI-900-008?
During the develomment of 17ß-Estradiol ELISA kit (ADI-900-008), the cross reactivities for a number of related steroid compounds was determined…
Tue, Jul 18, 2023

Does 17ß-Estradiol ELISA kit, ADI-900-008 cross react with Estradiol 3-benzoate?
Estradiol 3-benzoate has not been determined for cross reactivity with17ß-Estradiol ELISA kit (ADI-900-008). The cross relativities for a number of…
Tue, Jul 18, 2023

27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023

Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023

Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023

What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023

Can results from SARS-CoV-2 IgG ELISA Kit ENZ-KIT170 be correlated to plaque-forming units (pfu/ml) of SARS-CoV-2?
SARS-CoV-2 IgG ELISA Kit is designed for the qualitative measurement of the human SARS-CoV-2 IgG. ELISA detects human antibodies to COVID. …
Tue, Jul 18, 2023