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ELISA Immunoassays
How are the capture anti-HCP antibodies produced for CHO host cell protein ELISA kit, ENZ-KIT128?
The anti-HCP antibodies used in CHO HCP ELISA kit, ENZ-KIT128 are produced from rabbit serum and affinity purified using protein…
Tue, Jul 18, 2023
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
Are the immunogens used to produce anti-HCPs antibodiesin the CHO host cell protein ELISA kit (ENZ-KIT128) derived from the cell culture supernatant or extracted proteins from CHO cells?
The antigens used to produce anti-HCPs antibodies for CHO host cell protein ELISA kit, ENZ-KIT128 are purified from the conditioned…
Tue, Jul 18, 2023
What could be the reasons for lower spike and recovery results with Protein A ELISA kit, ADI-900-057?
There are several reasons for lower spike and recovery return values with Protein A ELISA kit (ADI-900-057). One possible reason…
Tue, Jul 18, 2023
How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
What is the difference between Prostaglandin E2 high sensitivity ELISA kit, ADI-930-001 and PGE2 ELISA kit, ADI-900-001?
The PGE2 high sensitivity ELISA kit with catalog number ADI-930-001 was specifically developed to quantify the levels of PGE2 in…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023
What are the Intra- assay and Inter-assay precision percent coefficient of variation for the concentrations between lots for E. coli host cell protein ELISA kit, ENZ-KIT127 ?
Concerning the E. coli host cell protein ELISA kit (ENZ-KIT127) our intra-assay %CV is
Tue, Jul 18, 2023
How do we perform QC for E. coli host cell protein ELISA kit, ENZ-KIT127?
Quality control for E. coli host cell protein ELISA kit (ENZ-KIT127) is performed by randomly selecting multiple plates from each…
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023