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ELISA Immunoassays
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
Are the immunogens used to produce anti-HCPs antibodiesin the CHO host cell protein ELISA kit (ENZ-KIT128) derived from the cell culture supernatant or extracted proteins from CHO cells?
The antigens used to produce anti-HCPs antibodies for CHO host cell protein ELISA kit, ENZ-KIT128 are purified from the conditioned…
Tue, Jul 18, 2023
What could be the reasons for lower spike and recovery results with Protein A ELISA kit, ADI-900-057?
There are several reasons for lower spike and recovery return values with Protein A ELISA kit (ADI-900-057). One possible reason…
Tue, Jul 18, 2023
What is the difference between Prostaglandin E2 high sensitivity ELISA kit, ADI-930-001 and PGE2 ELISA kit, ADI-900-001?
The PGE2 high sensitivity ELISA kit with catalog number ADI-930-001 was specifically developed to quantify the levels of PGE2 in…
Tue, Jul 18, 2023
How are the capture anti-HCP antibodies produced for CHO host cell protein ELISA kit, ENZ-KIT128?
The anti-HCP antibodies used in CHO HCP ELISA kit, ENZ-KIT128 are produced from rabbit serum and affinity purified using protein…
Tue, Jul 18, 2023
How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
What are the Intra- assay and Inter-assay precision percent coefficient of variation for the concentrations between lots for E. coli host cell protein ELISA kit, ENZ-KIT127 ?
Concerning the E. coli host cell protein ELISA kit (ENZ-KIT127) our intra-assay %CV is
Tue, Jul 18, 2023
How do we perform QC for E. coli host cell protein ELISA kit, ENZ-KIT127?
Quality control for E. coli host cell protein ELISA kit (ENZ-KIT127) is performed by randomly selecting multiple plates from each…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023