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ELISA Immunoassays
Competitive Immunoassays
In competitive ELISA Assays, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a…
Tue, Jul 18, 2023
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
Detection Methods for Immunoassays
The immunological part of an immunoassay ends typically with either a wash or separation step. In these steps, any unbound…
Tue, Jul 18, 2023
When is it appropriate to use a CLIA instead of an EIA?
A CLIA, or chemiluminescent immunoassay, is more sensitive than an EIA (enzyme immunoassay) and is used when samples contain very…
Tue, Jul 18, 2023
Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small…
Tue, Jul 18, 2023
Can you explain why I am seeing inconsistent standard curves run in culture media?
Often times, the serum supplemented into media can be problematic. In order to minimize this problem it is very important…
Tue, Jul 18, 2023
How do I know if I need to dilute or extract my samples?
If your samples contain analyte levels within the dynamic range of the assay at the minimum recommended dilution for your…
Tue, Jul 18, 2023
What species does your kit work with?
Kits where the molecule is conserved among all species (e.g. eicosanoids, steroids) are species independent, and may be used with…
Tue, Jul 18, 2023
Can I use a spectrophotometer to read my assay?
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting…
Tue, Jul 18, 2023
Do I have to use a wavelength correction when reading an ELISA plate?
Reading at dual wavelengths corrects for the optical density contributed by the plastic the plate wells are made of as…
Tue, Jul 18, 2023