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Cellular Analysis
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
What is the detailed nature of the Cyto-ID® autophagy dye and how does the assay work?
The precise chemical structure of the dye is proprietary. The probe is a cationic amphiphilic tracer (CAT) dye that rapidly…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023
Can the Cyto-ID® green detection dye be used in conjunction with another dye to differentiate between endosomes and amphisomes?
The Cyto-ID® Red long-term tracer dye, ENZ-51037- inserts into the plasma membrane and later is internalized by endocytosis. Cyto-ID® Red…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
Does the standard included in BML-AK555 need to be incubated with recombinant SIRT1?
The deacetylated standard included in the kit can be used to create a standard curve giving the relationship between deacetylated…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023