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Cellular Analysis
How soon after incubation with Cyto-ID® autophagy dye would the samples need to be analyzed by flow cytometry? The user manual recommends following 30 minutes incubation. Is it advisable to temporarily store samples on ice before they are analyzed?
One can store the samples on ice for a short period of time to extend the period of optimal staining.…
Tue, Jul 18, 2023
How long can the fluorescence signal be detected in live cells?
Typically, cells are induced to undergo autophagy and then stained just prior to analysis. Induction can be as little as…
Tue, Jul 18, 2023
For the ROS/RNS detection kit, ENZ-51001, can samples be stored after the final wash buffer step in the protocol, and do the microscopy at a later date?
No, this is a vital stain. Live cells must be read immediately per the manual.
Tue, Jul 18, 2023
How long can the Cyto-ID® green detection reagent be left incubating with live cells (mins/hours/days)?
We do not recommend to incubate cells with Cyto-ID® green detection reagent for longer that 30 minutes at 37°C. In…
Tue, Jul 18, 2023
Can GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID® Autophagy Detection Kit, ENZ-51031, be combined to perform triple staining simultaneously detecting apoptotic, necrotic and autophagic cells ?
GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID®Autophagy Detection Kit, ENZ-51031, cannot be used in combination because of spectral overlap between…
Tue, Jul 18, 2023
Using this kit, is it necessary to wash the cells after staining?
No, the kit does not require a wash step.
Tue, Jul 18, 2023
Can the Cyto-ID® green detection dye be used in conjunction with another dye to differentiate between endosomes and amphisomes?
The Cyto-ID® Red long-term tracer dye, ENZ-51037- inserts into the plasma membrane and later is internalized by endocytosis. Cyto-ID® Red…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
Which cell lines and primary cells/tissues have been tested with Cyto-ID® autophagy dye?
Some representative cells and cell lines tested with the kit include hepatocytes, SK-N-SH neuroblastoma cells, Chinese hamster ovary (CHO) cells,…
Tue, Jul 18, 2023
Little or no apoptosis observed or detected
None or few apoptotic cells present in sample >> Use the Apoptosis Inducer, provided in the kit, as a positive…
Tue, Jul 18, 2023