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Cellular Analysis
Is it possible to distinguish between protein aggregates of different sizes using ProteoStat® Protein Aggregation Assay, ENZ-51023?
The ProteoStat® detection dye does not differentiate aggregate sizes. The dye will bind to a wide range of protein aggregate…
Tue, Jul 18, 2023
Would any protein work with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027?
When a protein aggregates in response to thermal stress, then the ProteoStat®dye would detect the aggregates. The exact efficiency of detection…
Tue, Jul 18, 2023
Is the ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, compatible with the food proteins?
Beta-lactoglobulin is the control provided in the kit. In the food industry, protein aggregation is a desired attribute since aggregates impart…
Tue, Jul 18, 2023
Can Cyto-ID® Green autophagy detection reagent from Cyto-ID® Autophagy detection kit, ENZ-51031, be used in combination with ProteoStat® Detection Reagent in ProteoStat® Protein aggregation assay, ENZ-51023?
Since there is no spectral overlap between Cyto-ID® Green Autophagy Detection Reagent and ProteoStat® Detection Reagent, they can be used…
Tue, Jul 18, 2023
Is the binding of the protein to the ProteoStat® detection dye reversible?
The interaction between the ProteoStat® dye and the protein aggregates is non-covalent. So, in theory it is a reversible interaction.…
Tue, Jul 18, 2023
What are the critical points to pay attention to during preparation of ß-Lactoglobulin solution?
During preparation of ß-Lactoglobulin solution, the following points should be noted: The ß-Lactoglobulin Control is supplied as a lyophilized powder…
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023
What are the applications for ProteoStat® Thermal shift stability assay kit, ENZ-51027?
The ProteoStat® Thermal Shift Stability Assay kit (ENZ-51027) is used for direct monitoring of protein aggregation arising from a thermally-induced…
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
Cells do not exhibit fluorescence after incubation with the detection reagent.
Cell viability is low. Cells should be in log growth phase. Cell samples cannot be kept longer than 6 hours…
Tue, Jul 18, 2023