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Cellular Analysis
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023
Is it possible to detect changes in calcium level in cells nuclei following specific treatment with the FLUOFORTE® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020)?
Detecting the calcium level in cells nuclei would require optimization. FLUOFORTE® Calcium Assay Kits (ENZ-51016, ENZ-51017, ENZ-51020) have been benchmarked for…
Tue, Jul 18, 2023
Is the CYTO-ID® Long Term Tracer Dye (ENZ-51036, ENZ-51037) toxic to cells?
Our CYTO-ID long-term cell tracer kits (ENZ-51036, ENZ-51037) use non-covalent cell labeling technology to stably incorporate the fluorescent dye containing…
Tue, Jul 18, 2023
Can the CYTO-ID® dye (ENZ-51036, ENZ-51037) be injected into mice for in vivo studies or does it need to be loaded into cultured cells?
CYTO-ID® long-term cell tracer kits (ENZ-51036, ENZ-51037) have not been tested for in vivo studies. The CYTO-ID dye would be difficult…
Tue, Jul 18, 2023
What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023
Do you offer different colored dyes with the same binding capacity to dsDNA?
We offer red, blue or green fluorescent dyes suitable for dsDNA binding, as a stand alone or as part of…
Tue, Jul 18, 2023
How long is the NUCLEAR-ID® red dye (ENZ-52406) retained by the cells and is the dye toxic to the cells?
NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be…
Tue, Jul 18, 2023
Is it possible to combine FLUOFORTE® Calcium Assay Kit (ENZ-51016) with other fluorescence dye in a multiplex assay? What is the fluorescence excitation and emission of the dye?
It is possible to multiplex FLUOFORTE® Calcium Assay Kit with other assays as long as there is no spectral overlap…
Tue, Jul 18, 2023
Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023
How specific is the NUCLEAR-ID® red dye (ENZ-52406) for dsDNA? What is the binding capacity of the dye to ssRNA, ssDNA and DNA:RNA Hybrids?
The dye stains dsDNA. Data is not available for binding capacity to ssRNA, ssDNA and DNA:RNA hybrids.
Tue, Jul 18, 2023