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Cellular Analysis
What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023
Can you provide an overview of cell proliferation experiments with the CYTO-ID® long-term cell tracer kits (ENZ-51036, ENZ-51037)?
It is possible to kinetically monitor the ability of the cells to proliferate with CYTO-ID® Red long-term cell tracer kit…
Tue, Jul 18, 2023
Can the CYTO-ID® dye (ENZ-51036, ENZ-51037) be injected into mice for in vivo studies or does it need to be loaded into cultured cells?
CYTO-ID® long-term cell tracer kits (ENZ-51036, ENZ-51037) have not been tested for in vivo studies. The CYTO-ID dye would be difficult…
Tue, Jul 18, 2023
Is the CYTO-ID® Long Term Tracer Dye (ENZ-51036, ENZ-51037) toxic to cells?
Our CYTO-ID long-term cell tracer kits (ENZ-51036, ENZ-51037) use non-covalent cell labeling technology to stably incorporate the fluorescent dye containing…
Tue, Jul 18, 2023
How long is the CYTO-ID® long-term cell tracer dye (ENZ-51036, ENZ-51037) retained in the cell and what cells were used to validate the assay?
The dye is well retained by cells for up to 96-120 hours after loading depending on how fast the cells…
Tue, Jul 18, 2023
Is it possible to detect changes in calcium level in cells nuclei following specific treatment with the FLUOFORTE® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020)?
Detecting the calcium level in cells nuclei would require optimization. FLUOFORTE® Calcium Assay Kits (ENZ-51016, ENZ-51017, ENZ-51020) have been benchmarked for…
Tue, Jul 18, 2023
Is it possible to combine FLUOFORTE® Calcium Assay Kit (ENZ-51016) with other fluorescence dye in a multiplex assay? What is the fluorescence excitation and emission of the dye?
It is possible to multiplex FLUOFORTE® Calcium Assay Kit with other assays as long as there is no spectral overlap…
Tue, Jul 18, 2023
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
What is the structure of the FluoForte dye and how does the dye detect Calcium levels in the cell?
The fluorogenic calcium-binding dye is a proprietary acetoxymethyl (AM) ester, which is cell-permeable. Once the dye enters the cells, it…
Tue, Jul 18, 2023