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Cellular Analysis
How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?
Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…
Tue, Jul 18, 2023
What is the recommendation for dye to cell ratio for microscopy and flow cytometry staining with the MITO-ID® Membrane potential detection kit, ENZ-51018?
For detailed information on the protocol, please follow the indications provided in the manual avalable from download on MITO-ID® Membrane…
Tue, Jul 18, 2023
Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?
The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…
Tue, Jul 18, 2023
Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?
Staining of yeast cells with Mito-ID® Red detection kit has not been tested. The kit could work in yeast cells with optimization…
Tue, Jul 18, 2023
What is the mechanism of action for LYSO-ID® detection dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 are proprietary cationic amphiphilic tracers (CAT) that rapidly and selectively stains acidic…
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
How long is the CYTO-ID® long-term cell tracer dye (ENZ-51036, ENZ-51037) retained in the cell and what cells were used to validate the assay?
The dye is well retained by cells for up to 96-120 hours after loading depending on how fast the cells…
Tue, Jul 18, 2023
Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023
What is the structure of the FluoForte dye and how does the dye detect Calcium levels in the cell?
The fluorogenic calcium-binding dye is a proprietary acetoxymethyl (AM) ester, which is cell-permeable. Once the dye enters the cells, it…
Tue, Jul 18, 2023
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023