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Cellular Analysis
Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023
Have the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 been tested and used in clinical trial samples such as PBMC, bone marrow samples?
We have not used EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) with clinical samples and we are not aware if…
Tue, Jul 18, 2023
Does the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 kits work with frozen samples?
The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for live cell analysis. If cells are healthy upon…
Tue, Jul 18, 2023
How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?
Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…
Tue, Jul 18, 2023
What is the recommendation for dye to cell ratio for microscopy and flow cytometry staining with the MITO-ID® Membrane potential detection kit, ENZ-51018?
For detailed information on the protocol, please follow the indications provided in the manual avalable from download on MITO-ID® Membrane…
Tue, Jul 18, 2023
Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?
The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…
Tue, Jul 18, 2023
Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?
Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…
Tue, Jul 18, 2023
Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
What is the chemical identity of the LYSO-ID® dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
The precise chemical identity of the LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 is proprietary. These are a cell…
Tue, Jul 18, 2023