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What species does your kit work with?
Kits where the molecule is conserved among all species (e.g. eicosanoids, steroids) are species independent, and may be used with…
Tue, Jul 18, 2023
Can I use a spectrophotometer to read my assay?
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting…
Tue, Jul 18, 2023
Do I have to use a wavelength correction when reading an ELISA plate?
Reading at dual wavelengths corrects for the optical density contributed by the plastic the plate wells are made of as…
Tue, Jul 18, 2023
What is the minimum recommended dilution for samples? Can I dilute less?
This is the minimum dilution required to eliminate matrix interference in the assay. In general, this data is collected using…
Tue, Jul 18, 2023
What is actually being stained in the nucleolus by the Nucleolar-ID™ Green Detection Reagent?
We use an RNA binding dye and this has been validated. However, in the context of live cells we cannot…
Tue, Jul 18, 2023
Detection Methods for Immunoassays
The immunological part of an immunoassay ends typically with either a wash or separation step. In these steps, any unbound…
Tue, Jul 18, 2023
Can you explain why I am seeing inconsistent standard curves run in culture media?
Often times, the serum supplemented into media can be problematic. In order to minimize this problem it is very important…
Tue, Jul 18, 2023
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
Competitive Immunoassays
In competitive ELISA, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a reporter…
Tue, Jul 18, 2023
Immunometric Assays
Immunometric assays, also known as sandwich assays, use two antibodies specific to the antigen to capture or “sandwich” antigen in…
Tue, Jul 18, 2023