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Knowledge Base
Blocking Peptide Neutralization
Reconstitute the lyophilized peptide with 0.1 mL distilled water or PBS. Be careful to reconstitute entire contents of vial as…
Tue, Jul 18, 2023
Western Blot Protocol
1. Transfer proteins from gel to membrane (nitrocellulose or PVDF). Gloves should be worn when handling gels or blot membrane.…
Tue, Jul 18, 2023
Immunoprecipitation of Phosphoserine-, Phosphothreonine- and Phosphotyrosine-Containing Proteins
Important Remarks: Rinse all plastic vessels with BSA solution (10 mg/mL in lysis buffer). Use lipid-free BSA, e.g., Sigma A7906. For…
Tue, Jul 18, 2023
Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
What storage conditions are needed for an antibody?
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage recommendations are listed on the…
Tue, Jul 18, 2023
Applications
Abbreviation Full Name AA Activity Assay AC Affinity Chromatography CIE Crossed Immune Electrophoresis DB Dot Blot…
Tue, Jul 18, 2023
General Abbreviations
Abbreviation Full Name mAb Monoclonal Antibody pAb Polyclonal Antibody cAb Clonal Antibody CT C-terminus NT …
Tue, Jul 18, 2023