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Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023
Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA Kit is used. An advantage of this assay…
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
What storage conditions are needed for an antibody?
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage recommendations are listed on the…
Tue, Jul 18, 2023
General Abbreviations
Abbreviation Full Name mAb Monoclonal Antibody pAb Polyclonal Antibody cAb Clonal Antibody CT C-terminus NT …
Tue, Jul 18, 2023
Non-Antibody Based Assays
Activity Assays Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring…
Tue, Jul 18, 2023