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Knowledge Base
Western Blot Protocol
1. Transfer proteins from gel to membrane (nitrocellulose or PVDF). Gloves should be worn when handling gels or blot membrane.…
Tue, Jul 18, 2023
Membrane Preparation Protocol
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA Kit is used. An advantage of this assay…
Tue, Jul 18, 2023
Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023
Antibody Conjugation
Abbreviation Full Name PE R-Phycoerythrin DY DYTM is a trademark of the Dyomics , Inc. DyLightTM 488 …
Tue, Jul 18, 2023
Applications
Abbreviation Full Name AA Activity Assay AC Affinity Chromatography CIE Crossed Immune Electrophoresis DB Dot Blot…
Tue, Jul 18, 2023
General Immunoprecipitation Protocol
Immunoprecipitation (IP) is a common useful immunochemical technique. When coupled with SDS-PAGE, IP can reveal a number of important characteristics…
Tue, Jul 18, 2023
Immunofluorescence Protocol
Principle Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023