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Knowledge Base
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
Does PACOCF3, BML-ST336 inhibit all isoforms of phospholipase A2 (PLA2)?
PACOCF3 (BML-ST336) inhibits of both Ca2+-dependent cytosolic cPLA2 (IC50=45 µM) and Ca2+-independent phospholipases A2 iPLA2 (IC50=3.8 µM). Unfortunately, we have…
Tue, Jul 18, 2023
What is the size of ENZO PATHO-GENE® HPV probes and what regions do they target?
All of the PATHO-GENE® HPV probe we offer, vary in size from 100 bp to 1 Kb and the probe…
Tue, Jul 18, 2023
What is the recommended solvent to dissolve Microcystin-LR, ALX-350-012 and what is the maximum solubility?
Maximum solubility of Microcystin-LR (ALX-350-012) is 10 mg/ml in 100 % ethanol, methanol or DMSO.
Tue, Jul 18, 2023
What could be the reasons for lower spike and recovery results with Protein A ELISA kit, ADI-900-057?
There are several reasons for lower spike and recovery return values with Protein A ELISA kit (ADI-900-057). One possible reason…
Tue, Jul 18, 2023
How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
What is the recommended final concentration of Z-Gly-Gly-Leu-AMC, BML-ZW8505 in a protease assay?
Z-Gly-Gly-Leu-AMC is added to a final concentration of 200 µM for solution based enzyme assays. For cell based assays, concentration of…
Tue, Jul 18, 2023
Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023
What is the recommended solvent to dissolve Z-Gly-Gly-Leu-AMC, BML-ZW8505?
Z-Gly-Gly-Leu-AMC, BML-ZW8505-0005 can be dissolved in methanol. Maximum solubility is 10 mg/ml to obtain a clear colorless solution
Tue, Jul 18, 2023