AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Knowledge Base
When using an IMMUNOSET® ELISA development set, can I use a different buffer to dilute my standards?
Use of buffers other than those recommended will require validation by the user.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, what kind of substrate should I use to develop the plate?
Any colorimetric peroxidase substrate compatible with the enzyme of choice as well as your plate reader should be acceptable. See…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use a chemiluminescent substrate for more sensitivity?
Use of a chemiluminescent substrate is not validated and may require additional assay optimization.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how long can I store the components after I reconstitute them?
The reconstituted reagents should be aliquotted and stored at the temperature recommended in the instruction manual for up to three…
Tue, Jul 18, 2023
Can I measure tissue NO with the Nitric Oxide detection kits?
Yes. Samples can be homogenized in the provided Reaction Buffer and then centrifuged. The supernatant should then be filtered through…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use other Streptavidin-HRP solutions?
Yes, however the reagent should be optimized (titered) to assure acceptable signal intensity and background are achieved.
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023