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Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023
I am using the Direct 8-iso PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
At this point, you have added HCl to NaOH which will yield NaCl + H2O. There is little buffering capacity…
Tue, Jul 18, 2023
What antibody pairs are used in the kit?
This varies from kit to kit. Refer to the product specific instruction manual for more information.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I prepare a different standard range than what is recommended in the protocol?
You may prepare a standard range to suit your specific needs. However, the dynamic range has already been optimized, and…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, should I store the coated plates wet or dry?
You may store the coated plates for short periods of time (2-3 days) in blocking buffer, being careful to seal…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will my plate coating technique affect the precision of my plates and the overall assay?
It is possible for plate coating technique to lead to poor precision. Please refer to the insert for specific recommendations.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I get the greatest stability out of the coated plates?
After blocking, shake out all excess solution and store desiccated at 4°C.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I avoid evaporation of the solutions while coating, blocking, and assaying?
Seal the plates with a plate sealer, plastic wrap, or foil during incubations.
Tue, Jul 18, 2023
How do I know whether to use the Nitric Oxide (NO<sub>2-</sub>/NO<sub>3-</sub>) detection kit or the Nitric Oxide (total) detection kit?
The regular kit measures both major metabolic products of NO — nitrate and nitrite. The Total kit measures these metabolites…
Tue, Jul 18, 2023