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What type of 10,000 MWCO filter is best to use prior to using the Nitric Oxide detection kits?
The user has a choice between a spin-filter, a syringe-type filter, or a 96-well filtration plate format. Generally, a filter…
Tue, Jul 18, 2023
Why do I have to filter my samples through a 10,000 MWCO filter prior to using the Nitric Oxide detection kits?
In order to get the best sensitivity, samples need to be filtered to remove or reduce interfering substances. Using a…
Tue, Jul 18, 2023
My cell culture samples are in DMEM. Will this interfere with the Nitric Oxide assay?
Yes. The nitrate salt used in this particular media will cause interference. It is important to check the published media…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023
Can I measure tissue NO with the Nitric Oxide detection kits?
Yes. Samples can be homogenized in the provided Reaction Buffer and then centrifuged. The supernatant should then be filtered through…
Tue, Jul 18, 2023
How do I know whether to use the Nitric Oxide (NO<sub>2-</sub>/NO<sub>3-</sub>) detection kit or the Nitric Oxide (total) detection kit?
The regular kit measures both major metabolic products of NO — nitrate and nitrite. The Total kit measures these metabolites…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how long can I store the components after I reconstitute them?
The reconstituted reagents should be aliquotted and stored at the temperature recommended in the instruction manual for up to three…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use a chemiluminescent substrate for more sensitivity?
Use of a chemiluminescent substrate is not validated and may require additional assay optimization.
Tue, Jul 18, 2023