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Knowledge Base
Can the 17ß-Estradiol ELISA kit be used with samples from any species?
17ß-Estradiol is conserved across all species and the antibody provided in the kit will bind to 17ß-Estradiol independently from the…
Tue, Jul 18, 2023
How should I treat my samples with the steroid displacement reagent (SDR) for analysis with the 17ß-Estradiol ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: 1µl of SDR per 99µl…
Tue, Jul 18, 2023
My sample type is not listed in your insert. Can the kit be used anyway?
All sample types that have been positively tested are indicated on our website in the field “Application” and in the…
Tue, Jul 18, 2023
What is the minimum sample volume for using the kit?
The assay requires 100 µL of diluted sample per well. The amount needed, therefore, depends on the samples themselves and…
Tue, Jul 18, 2023
If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023
Would membrane proteins work with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat® Detection Reagent in ProteoStat® Protein Aggregation Assay, ENZ-51023?
We have not tested the ProteoStat® Protein Aggregation Assay, ENZ-51023, or ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, with membrane…
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
How should I test for possible matrix interference with my samples?
Matrix interference describes the situation where the sample matrix (buffer salts, pH, additives like detergents or solvents, proteins, …) interferes…
Tue, Jul 18, 2023