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How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023
How should urine samples be prepared for use with the NGAL (human) ELISA kit?
Urine preparation usually only requires centrifugation if there is inflammation or infection of the urinary tract (to remove polymorphonuclear neutrophils…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
How should serum samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use…
Tue, Jul 18, 2023
Will my assay results vary depending on the sample type I am going to analyze with the NGAL (human) ELISA kit?
Analyses of samples of serum, EDTA plasma, citrate plasma, heparin plasma or urine showed no significant differences in analytical recovery,…
Tue, Jul 18, 2023
How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
It appears that the positive control is not working properly. What could be the reason?
The nature of that protein is that it can quickly degrade unless it is handled very carefully. It must be…
Tue, Jul 18, 2023
What is the principle of the PKA kinase activity assay?
The PKA kinase activity kit allows relative quantification of PKA kinase activity in different sample types (purified, partially purified and…
Tue, Jul 18, 2023