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Will the NGAL (human) ELISA kit also work with species other than human?
This kit has been solely developed and validated for human samples. Please note however that Enzo has several other NGAL ELISA kits…
Tue, Jul 18, 2023
Will my assay results vary depending on the sample type I am going to analyze with the NGAL (human) ELISA kit?
Analyses of samples of serum, EDTA plasma, citrate plasma, heparin plasma or urine showed no significant differences in analytical recovery,…
Tue, Jul 18, 2023
How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023
How should urine samples be prepared for use with the NGAL (human) ELISA kit?
Urine preparation usually only requires centrifugation if there is inflammation or infection of the urinary tract (to remove polymorphonuclear neutrophils…
Tue, Jul 18, 2023
How should serum samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
Does the kit work for species other than human?
The ADI-EKS-390A kit is actually species independent; therefore, as long as there is sufficient PKA activity in your samples and…
Tue, Jul 18, 2023
Can I use this kit to measure the concentration of PKA in my samples?
The kit does not measure the concentration of PKA in samples. It is intended for looking at relative PKA kinase activity.
Tue, Jul 18, 2023