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How stable are my samples prior to HSP70 quantification?
Different storage conditions for samples have not been analyzed or compared. If samples have been stored at -20°C or below…
Tue, Jul 18, 2023
Is HSP70B’; simply a variant of HSP70?
HSP70B’ is a product of a separate gene, which is devoid of introns similarly to HSP70. The HSP70B’ has 77%…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023
What are your recommendations as for mounting media and post-processing for your HIGHDEF® chromogens?
All our chromogens should be used preferentially with an aqueous-based mounting medium (e.g. ADI-950-261) for a better resolution and a…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
What is the difference between ADI-950-140 and ADI-950-141?
Some red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic where an automated slide strainer is used. The…
Tue, Jul 18, 2023
Why is the SAVIEW® PLUS detection technology superior compared to the traditional ABC detection method?
The high affinity of the non-covalent interaction between biotin and streptavidin (Kd 4×10-14 M) forms the basis for our SAVIEW®…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023