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Knowledge Base
Which species can be analyzed with the LTB4 ELISA kit?
Leukotriene B4 (LTB4) is a small molecule and thus a defined chemical entity that does not change in between species…
Tue, Jul 18, 2023
Which C18 reverse phase columns can be used for sample extraction with the LTB4 ELISA kit?
The Honeywell Burdick & Jackson® Non-Polar Silica Based SPE Columns, Cat. No. 9002, is the recommended C18 reverse phase column for…
Tue, Jul 18, 2023
When do I need to extract my samples for use with the LTB4 ELISA kit?
Sample extraction for this assay may only be needed if you have low LTB4 levels, so your samples cannot be…
Tue, Jul 18, 2023
How to prevent the formation of bubbles when using the HIGHDEF® IHC mount (ADI-950-261)?
In order to prevent bubble formation, please follow these recommendations:1) Remove red tip from the bottle and cut the tip off…
Tue, Jul 18, 2023
What is the difference between the mounting media ADI-950-260 and ADI-950-261?
HIGHDEF® IHC fluoromount (ADI-950-260) was specifically designed as an antifade reagent to cover tissues stained with fluorescent dyes. HIGHDEF® IHC…
Tue, Jul 18, 2023
What are your recommendations as for mounting media and post-processing for your HIGHDEF® chromogens?
All our chromogens should be used preferentially with an aqueous-based mounting medium (e.g. ADI-950-261) for a better resolution and a…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
What is the difference between ADI-950-140 and ADI-950-141?
Some red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic where an automated slide strainer is used. The…
Tue, Jul 18, 2023
Why is the SAVIEW® PLUS detection technology superior compared to the traditional ABC detection method?
The high affinity of the non-covalent interaction between biotin and streptavidin (Kd 4×10-14 M) forms the basis for our SAVIEW®…
Tue, Jul 18, 2023