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I expect calcineurin to be mainly located in the nuclei. How should I treat my cell lysates in order to avoid loss of nuclei whe using Calcineurin cellular activity assay kit, BML-AK816?
You need to replace the high speed centrifugation suggested in the Calcineurin cellular activity assay kit (BML-AK816) manual which pellets…
Tue, Jul 18, 2023
Can the kit BML-AK804, Calcineurin Phosphatase assay kit be used for biological samples?
The product BML-AK804, Calcineurin Phosphatase assay kit is designed for inhibitor screening or kinetic assays using the recombinant Calcineurin enzyme…
Tue, Jul 18, 2023
Do light or divalent cations such as Ca2+ or Mg2+ interfere with NO measurement by DAF-2?
M. Broillet, et al (2001) and J. Chatton, et al (2002) reported that light and divalent cations, such as Ca2+…
Tue, Jul 18, 2023
Does DAF-2 react with ascorbic acid?
X. Zhang, et al. (2002) reported that DAF-2 reacted with dehydroascorbic acid (DHA) and ascorbic acid (AA) to generate fluorescent…
Tue, Jul 18, 2023
What is the recommend Calmodulin concentration to fully activate Calcinuerin in the Calcineurin Phosphatase assay kit, BML-AK804?
We provide 25µM Calmodulin with the Calcineurin Phosphatase assay kit (BML-AK804) and recommend a concentration of 0.5µM to fully activate…
Tue, Jul 18, 2023
Can the desalting resin provided with the kit BML-AK816, Calcineurin cellular activity assay kit be reused?
Yes, the desalting resin provided with the Calcineurin cellular activity assay kit (BML-AK816) can be reused for multiple samples. Between…
Tue, Jul 18, 2023
In the manual for the Calcineurin cellular activity assay kit, BML-AK816, you recommend using 5,000-50,000 cells per assay point. How much lysis buffer should be used to prepare the samples?
it is easier to dilute samples than to concentrate them. In order to prepare cells for use with Calcineurin cellular…
Tue, Jul 18, 2023
What is the difference between storing a product at -70°C and -80°C?
Products with instructions to be stored at either of these temperatures can be stored at =-70°C. Storage at -70°C or…
Tue, Jul 18, 2023
What is the difference between low- and high-density arrays?
Low-density arrays are usually prepared on glass slides with an average density of about 102 spots per cm2. High-density arrays…
Tue, Jul 18, 2023
How have the results you show on a patient with Prader-Willi syndrome been generated?
These results have been generated by Enzo scientists using the CYTAG™ CGH Labeling kit on an Agilent 4X180K oligonucleotide microarray.
Tue, Jul 18, 2023