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For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
Is there a way to quickly check for the labeling efficiency of Enzo’s CGH labeling kits?
Visual inspection after combining the Cyanine 3-labeled and Cyanine 5-labeled and cleaned DNA should be visibly purple. More detailed analysis…
Tue, Jul 18, 2023
What are the molar extinction coefficients of the Cyanine 3 and Cyanine 5 dyes in Enzo’s CGH labeling kits?
Cyanine 3: 150,000 M cm-1; Cyanine 5: 250,000 M cm-1
Tue, Jul 18, 2023
What are the key advantages of Enzo’s CGH labeling kits in comparison to the kits Agilent offers?
All our CGH labeling kits outperform the kits offered by Agilent for labeling: They provide excellent DNA yields with superior…
Tue, Jul 18, 2023
How does the BIOARRAY™ Terminal labeling kit, ENZ-42630, label nucleotides?
When using the BIOARRAYTMTerminal labeling kit, the Terminal Deoxynucleotide Transferase catalyzes the additional single biotin-ddUMP to the 3’-OH terminus of…
Tue, Jul 18, 2023
Can the BIOARRAY™ Terminal labeling kit (ENZ-42630) be used to label sequences of 100 nucleotides?
The BIOARRAYTM Terminal labeling kit kit was optimized with a 30-mer at 1 µg or 100 pmol, but a longer…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
Do I really need to add Stop Buffer after the labeling reaction with Enzo’s CGH labeling kits even though the enzyme is heat-inactivated?
During kit validation, a protocol utilizing a 4-hour labeling step and addition of Stop Buffer gave us the best results…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be handled?
We recommend aliquoting the kit reagents upon first use into volumes convenient for your workflow, and to avoid repeated freezing…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023