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What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?
Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…
Tue, Jul 18, 2023
Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
How to cover up the sections during hybridization in ISH protocols?
The most common method is to cover tissue sections on glass slides in the hybridization solution with a glass cover…
Tue, Jul 18, 2023
Is protein blocking really necessary in ISH?
Blocking with bovine serum albumin (BSA), milk or serum is always recommended when ISH signals are detected by antibodies (e.g.…
Tue, Jul 18, 2023
Why is peroxidase blocking necessary in colorimetric ISH/IHC detection?
When colorimetric detection by enzymatic reaction is used, a blocking step with peroxide solution is required to eliminate endogenous peroxidase…
Tue, Jul 18, 2023
Why are peptidases/proteinases used in ISH?
Peptidases or proteinases like proteinase K, pepsin or trypsin are used in ISH to digest proteins contained in the sample…
Tue, Jul 18, 2023
How important are denaturation and hybridization temperature in ISH?
Correct denaturation and hybridization temperatures are paramount for successful ISH. Regardless of the probe or target being RNA or DNA,…
Tue, Jul 18, 2023
How big are ISH probes?
There is no defined answer. Depending on the tissue specimen, target type, and sequence, ISH probes might vary from below…
Tue, Jul 18, 2023
What are ISH probes typically labeled with?
Common labels include biotin, digoxigenin, and fluorochromes. Biotin is detected with streptavidin- conjugated enzymes followed by an enzymatic colorimetric reaction.…
Tue, Jul 18, 2023
How are ISH probes labeled?
There are three main techniques for probe labeling. Labeled probes can be generated by incorporating labeled nucleotides during the synthesis…
Tue, Jul 18, 2023