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Knowledge Base
What is the recommended ratio of dTTP to fluorophore-dUTP when using the Nick translation DNA labeling system 2.0?
The SEEBRIGHT® fluorophore-labeled dUTPs are supplied as a 1 mM solution. We recommend to prepare working concentration of 0.3 mM…
Tue, Jul 18, 2023
What are the correction factors for measuring dye incorporation in a NanoDrop?
The correction factors for 260/280nm detection are as follows: Correction Factor at Aqua 431 Green 496 Green 500 Gold 525 Gold 500 Orange…
Tue, Jul 18, 2023
What are the spectra of the different fluorophore-labelled dUTPs?
The individual spectra of each SEEBRIGHT® fluorescent dUTP can additionally be found on the individual product’s webpage. Excitation and emission…
Tue, Jul 18, 2023
Why are Aqua 431 dUTP-labeled probes much harder to detect?
There are two aspects making Aqua-labeled probes harder to detect: First, the aqua fluorophore (DEAC – 7-Diethylaminocoumarin-3-carboxylic acid) has a…
Tue, Jul 18, 2023
Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
In which cases do you recommend to digest the DNA prior to labeling with Enzo’s CGH labeling kits?
For CGH+SNP arrays, genomic DNA must be digested using a combination of the restriction enzymes AluI and RsaI. For CGH…
Tue, Jul 18, 2023
What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
Apart from the labeling kits, which ancillary reagents does Enzo offer for CGH?
Enzo does provide reagents for restriction, labeling and purification of DNA and Cot DNA. Furthermore, ENZO provides control Human Genomic…
Tue, Jul 18, 2023
When I opened the parcel containing the CGH labeling kit from Enzo, all of the dry ice had disappeared, will it still be good to use?
If the vials in the kit were still frozen when you received them then the product is okay to use.…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023