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Bright yellow array with reduced magnitude of expected changes
This can be due to insufficient Cot-1 DNA, blocking agents or hybridization and washing conditions that are not stringent enough.
Tue, Jul 18, 2023
Good DLRs in the CGH analytics QC report, but some oligos show whiskering or significant deviation from the expected log2 ratio
We suggest keeping the Cot-1 DNA at 50 µg regardless of the hybridization volume.
Tue, Jul 18, 2023
Can the Cyanine 3- and Cyanine 5-labeled dUTPs be replaced by other Cyanine-labeled nucleotides?
As with any other kit, components of the CGH labeling kit for oligo arrays cannot be replaced by any other…
Tue, Jul 18, 2023
Can samples be frozen before clean up?
No. Samples should be stored at +4 °C after the addition of the stop buffer. They should not be frozen,…
Tue, Jul 18, 2023
Can this kit be used for labeling DNA for application to the Affymetrix’ aCGH platform?
This is not possible. The Affymetrix platform uses a biotin/streptavidin system with subsequent phycoerythrin-based detection, while Enzo’s CGH labeling kit…
Tue, Jul 18, 2023
How should the DNA be purified after labeling with Enzo’s CGH labeling kits?
Enzo's PCR & Gel Clean-up columns (ENZ-GEN100) are strongly recommended in this step. Also, Qiagen's QIAquick purification columns and buffers…
Tue, Jul 18, 2023