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Knowledge Base
Have any of Enzo Live Cell Fluorescent probes been validated on 3D spheroid culture?
While we did not specifically tested our Live Cell Analysis products on 3D models, we do have a few application…
Tue, Jul 18, 2023
Is it possible to multiplex MITO-ID® Membrane Potential Detection Kit, ENZ-51018 with NUCLEAR-ID® Blue/Red Cell Viability Reagent (GFP-CERTIFIED®), ENZ-53005?
MITO-ID/NUCLEAR-ID (ENZ-51018/ENZ-53005) multiplex assay have been multiplexed in a 3D spheroid cultures of iPSC-derived human hepatocytes (iCell Hepatocytes 2.0) assay…
Tue, Jul 18, 2023
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023
Liver lysate samples when probed with HO-1 polyclonal antibody, ADI-SPA-896, shows two bands on western blot. What is the possible explanation as to why two bands detected?
Western blot image displayed on product page with ADI-SPA-896 shows two bands being detected. One of them is full length…
Tue, Jul 18, 2023
How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023
How do we define Limit of Detection (LoD) and what is the percent reliability detection with AMPIPROBE® SARS-CoV-2 Assay Kit, ENZ-GEN215?
The LoD for AMPIPROBE® SARS-CoV-2 Assay Kit (ENZ-GEN215 ) is defined from this study as the lowest concentration of SARS-CoV-2…
Tue, Jul 18, 2023
Can PAP-AMC, Tyrosinase substrate, BML-EI405 function in live cell assay?
PAP-AMC, Tyrosinase substrate, BML-EI405 has been used in vitro assays. We do not have information if this substrate works efficiently…
Tue, Jul 18, 2023