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Is it possible to multiplex MITO-ID® Membrane Potential Detection Kit, ENZ-51018 with NUCLEAR-ID® Blue/Red Cell Viability Reagent (GFP-CERTIFIED®), ENZ-53005?
MITO-ID/NUCLEAR-ID (ENZ-51018/ENZ-53005) multiplex assay have been multiplexed in a 3D spheroid cultures of iPSC-derived human hepatocytes (iCell Hepatocytes 2.0) assay…
Tue, Jul 18, 2023
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
Can gene-specific primers be used for cDNA synthesis with BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421?
RNA amplification with BIOARRAYTM Single-round RNA amplification and biotin labeling system requires the use of primers included in the kit.…
Tue, Jul 18, 2023
Liver lysate samples when probed with HO-1 polyclonal antibody, ADI-SPA-896, shows two bands on western blot. What is the possible explanation as to why two bands detected?
Western blot image displayed on product page with ADI-SPA-896 shows two bands being detected. One of them is full length…
Tue, Jul 18, 2023
How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
How do we define Limit of Detection (LoD) and what is the percent reliability detection with AMPIPROBE® SARS-CoV-2 Assay Kit, ENZ-GEN215?
The LoD for AMPIPROBE® SARS-CoV-2 Assay Kit (ENZ-GEN215 ) is defined from this study as the lowest concentration of SARS-CoV-2…
Tue, Jul 18, 2023
How was the Limit of Detection (LoD) determined for AMPIPROBE® SARS-CoV-2 kit, ENZ-GEN215?
Preliminary LoD AMPIPROBE® for SARS-CoV-2 kit (ENZ-GEN215) for was determined testing triplicates of eight samples prepared by spiking the SeraCare…
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023