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The TNF-α (mouse), ELISA kit is a colorimetric immunometric enzyme immunoassay kit
- Stable color coded reagents and pre-coated plates reduce errors
- Assay up to 38 samples in just 5 hours
- Save time, money, and precious sample compared to Western blot analysis
The TNF-α (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 5 hours. Absorbance is read at 450 nm.
Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
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TNF-α (mouse), ELISA kit Kit graph
Product Details
| Alternative Name |
Tumor necrosis factor-α, TNFSF 2 |
|---|---|
| Application |
Colorimetric detection, ELISA |
| Application Notes |
For the quantitative determination of mouse TNF-α in culture supernatants and serum. Cited sample type includes tissue (ref. 1) and plasma. |
| Assay Time |
5 hours |
| Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
| Contents |
Microtiter plate, Conjugate, Antibody, Assay buffer 16, Wash buffer concentrate, Standard, TMB Substrate, Stop solution |
| Sensitivity |
3.9 pg/ml (range 15.63 – 2,000 pg/ml) |
| Species Reactivity |
Mouse |
| Technical Info / Product Notes |
Cited samples: |
| UniProt ID |
P06804 |
| Wavelength |
450 nm |
Handling & Storage
| Use/Stability |
Store all components at 4°C, except standard at -20°C. |
|---|---|
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Effect of Sardine and Sprat Thermal Processing on Intestinal Integrity and Macrophage Activation In Vitro.: Doskocil, I., Lampová, B., et al.; Foods 14, 3754 (2025), Abstract
- Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression: Kim, H. J., You, M. J., et al.; Front. Cell. Neurosci. 17, 1162966 (2023), Sample Type: Mouse serum, Abstract
- Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice: I.O. Shmarakov, et al.; Nat. Commun. 14, 1851 (2023), Sample Type: Mouse plasma and other (bronchioalveolar lavage fluid), Abstract
- Periodontitis promotes bacterial extracellular vesicle-induced neuroinflammation in the brain and trigeminal ganglion: Ha, J. Y., Seok, J., et al.; PLoS Pathog. 19, e1011743 (2023), Sample Type: Mouse tissue lysate (brain), Abstract
- Anti-inflammatory effects of new human histamine H3 receptor ligands with flavonoid structure on BV-2 neuroinflammation: Honkisz-Orzechowska, E., Popiołek-Barczyk, K., et al.; Inflamm. Res. 72, 181 (2023), Sample Type: Mouse culture supernatant (BV-2 microglial cell line), Abstract
- Exploring the therapeutic efficacy of Chlorella pyrenoidosa peptides in ameliorating Alzheimer’s disease: S.M. Wang, et al.; Heliyon 9, e15406 (2023), Sample Type: Mouse culture supernatant (N2A neuroblastoma cell line), Abstract
- Biflavonoid quercetin protects against cyclophosphamide–induced organ toxicities via modulation of inflammatory cytokines, brain neurotransmitters, and astrocyte immunoreactivity: A.Y. Onaolapo, et al.; Food Chem. Toxicol. 178, 113879 (2023), Sample Type: Rat other (blood), Abstract
- Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals 15, 287 (2022), Sample Type: Mouse plasma and tissue lysate, Abstract
- Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary: T. Umehara, et al.; Sci. Adv. 8, eabn4564 (2022), Sample Type: Mouse serum and culture supernatant (ovarian stromal cells), Abstract
- Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res. 66, e2200082 (2022), Sample Type: Mouse serum, Abstract
- Effects of Suberoylanilide Hydroxamic Acid (SAHA) on the Inflammatory Response in Lipopolysaccharide-Induced N9 Microglial Cells: Z.B. Çelik, et al.; Cureus 14, e32428 (2022), Sample Type: Mouse culture supernatant (N9 microglial cell line), Abstract
- BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res. 169, 105611 (2021), Sample Type: Human cell lysate (hepatocellular carcinoma cell lines), Abstract
- The Aging of Adipocytes Increases Expression of Pro-Inflammatory Cytokines Chronologically: Ahmed, B., Si, H., et al.; Metabolites 11, (2021), Sample Type: Mouse culture supernatant ( 3T3-L1 adipocyte cell line), Abstract
- Microglial activation elicits a negative affective state through prostaglandin-mediated modulation of striatal neurons: Klawonn, A. M., Fritz, M., et al.; Immunity 54, 225 (2021), Sample Type: Mouse serum, Abstract
- Emodin Promotes Autophagy and Prevents Apoptosis in Sepsis-Associated Encephalopathy through Activating BDNF/TrkB Signaling: Gao, L. L., Wang, Z. H., et al.; Pathobiology 89, 135 (2021), Sample Type: Mouse tissue lysate (brain [hippocampus]), Abstract
- 4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci. 37, 604 (2021), Sample Type: Mouse serum and tissue lysate (hind ancle joints), Abstract
- Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H. U. Shih, et al.; J. Pers. Med. 11, 436 (2021), Sample Type: Mouse tissue lysate (liver), Abstract
- Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants 10, 2209 (2021), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract
- Artemisia iwayomogi (Dowijigi) inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppressing the NF-κB signaling pathway: Kim, S. M., Vetrivel, P., et al.; Exp. Ther. Med. 19, 2161 (2020), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract
- The lymphocyte adapter protein: A negative regulator of myocardial ischemia/reperfusion injury: J. Huang, et al.; J. Mol. Cell. Cardiol. 134, 107 (2019), Sample Type: Mouse serum, Abstract
- Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway: Y. Lu, et al.; Hepatology 67, 1303 (2018), Sample Type: Mouse serum, Abstract — Full Text
- In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection: Y.H. Li, et al.; Int. J. Mol. Med. 41, 3620 (2018), Sample Type: Mouse serum, Abstract
- Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson’s disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol. 358, 10 (2018), Sample Type: Mouse tissue lysate (brain [substantia nigra]), Abstract
- Smad proteins differentially regulate obesity-induced glucose and lipid abnormalities and inflammation via class-specific control of AMPK-related kinase MPK38/MELK activity: H.A. Seong, et al.; Cell Death Dis. 9, 471 (2018), Sample Type: Mouse serum, Abstract — Full Text
- The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol. 18, 519 (2017), Sample Type: Mouse serum, Abstract
- Immunomodulation of cell-penetrating tat-metallothionein for successful outcome of xenotransplanted pancreatic islet: M.J. Kim, et al.; J. Drug Target. 25, 350 (2017), Sample Type: culture supernatant, Abstract
- Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract — Full Text
- The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother. 81, 111 (2016), Sample Type: Mouse plasma, Abstract
- CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Sample Type: Mouse serum and culture supernatant, Abstract
- Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Sample Type: Mouse plasma, Abstract — Full Text
- Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies: M. Abdel-Daim, et al.; Biomed. Pharmacother. 77, 79 (2016), Sample Type: Mouse serum, Abstract
- Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata: C.G. Lee, et al.; Int. J. Biol. Macromol. 85, 505 (2016), Sample Type: culture supernatant, Abstract
- Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract
- The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol. 31, 88 (2015), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract
- Interferon regulatory factor 9 is an essential mediator of heart dysfunction and cell death following myocardial ischemia/reperfusion injury: Y. Zhang, et al.; Basic Res. Cardiol. 109, 434 (2014), Sample Type: Mouse serum, Abstract
- Naltrexone attenuates endoplasmic reticulum stress induced hepatic injury in mice: A. Moslehi, et al.; Acta Physiol. Hung. 101, 341 (2014), Sample Type: Mouse tissue lysate (liver), Abstract
- Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Sample Type: Mouse culture supernatant (RAW 264.7 macrophage cell line), Abstract
- Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production: A. Nemec, et al.; Res. Vet. Sci. 92, 401 (2012), Sample Type: Mouse plasma, Abstract
- Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Sample Type: Mouse serum, Abstract — Full Text
- Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Sample Type: Mouse serum and culture supernatant, Abstract — Full Text
- Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al.; PNAS 105, 7670 (2008), Sample Type: Mouse tissue lysate (ovaries), Abstract
- Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al.; J. Immunol. 181, 2348 (2008), Sample Type: Mouse serum, Abstract
- Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Sample Type: Mouse tissue lysate (lung), Abstract — Full Text
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Last modified: November 5, 2025
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