LEXSY in vitro Translation Cell Extract

The LEXSY cell extract for in vitro translation contains functional ribosomes and all essential components of the eukaryotic translation and folding machinery. For target mRNA generation the heterologous T7 RNA polymerase was added to the extracts. To ensure a low background the translation of endogenous host mRNAs is efficiently blocked by an antisense oligonucleotide making use of the unique gene organization of Leishmania.