Human Amyloid Beta 1-40 (High Sensitivity) ELISA Kit

ELISA

Quantification of Aβ40 in plasma and serum for Alzheimer’s disease research. Always pair with ENZ-KIT273 (Aβ1-42) for Aβ42/40 ratio calculation. Suitable for APP processing studies and γ-secretase inhibitor assays. Primary validated matrices: serum and plasma. Other sample types should be verified prior to use.

3.5 hours

Standard 96-well microplate reader capable of reading absorbance at 450 nm.

Pre-Coated Microplate, 96 well (12 breakaway strips of 8); Standard (Lyophilized); Biotinylated Detection Antibody (100×); Streptavidin-HRP Conjugate (100×); Standard/Sample Diluent Buffer; Biotinylated Antibody Diluent; HRP Diluent; Wash Buffer (25×); TMB Substrate Solution; Stop Reagent; Adhesive Plate Covers

APP

1.46 pg/mL (Detection range: 4.69-300 pg/mL)

Human

Human Amyloid Beta 1-40 (High Sensitivity)

Two-step sequential sandwich ELISA. Capture antibody pre-coated on breakaway 96-well strips. Step 1: sample/standard incubation, 37°C, 80 min. Step 2: biotinylated detection antibody, 37°C, 50 min. Step 3: Streptavidin-HRP, 37°C, 50 min. Step 4: TMB substrate, 37°C, 20 min (protected from light). Stop reagent added; read at 450 nm. Intra-assay CV <8%; inter-assay CV <10%. Standard reconstituted in Standard/Sample Diluent Buffer. Do not mix reagents from different lot numbers (exception: TMB, Wash Buffer, Stop Reagent). Azide-free buffers required — azide inhibits HRP color development.

Detection range: 4.69–300 pg/mL. Sensitivity: 1.46 pg/mL. Plasma Aβ40: typically 150–250 pg/mL (stable between individuals). CSF Aβ40: typically 5,000–15,000 pg/mL in controls.

P05067

450 nm